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产物英文名称: WNT-5a, Human
产物中文名称:人重组奥狈罢-5补蛋白
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WNT-5a, Human产物介绍:
Wnt信号转导通路因其启动蛋白为Wnt蛋白而得名,在胚胎发育、细胞增殖及分化等方面发挥重要 作用,其异常活化与人类多种肿瘤的发生密切相关。研究显示,Wnt信号至少存在经典和非经典两条不同的信号通路,而Wnt-5a属于非经典Wnt信号通 路,既往研究Ror2具有Wnt受体结构域样结构。有研究认为Wnt-5a经由Ror2介导的 信号通路发挥作用。
Human WNT-5a蛋白含有357个氨基酸残基。
WNT-5a, Human 产物特性:
? 活性在共转Frizzled- 4 和 LRP- 5基因的HEK293 细胞鉴定,纯度高,无动物成分污染
? WNT-5a, Human活性在共转Frizzled- 4 和 LRP- 5基因的HEK293 细胞鉴定
? WNT-5a, Human产物经过SDS-PAGE 和HPLC双层鉴定纯度大于85%
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WNT-5a, Human产物英文简述:
WNT-5a belongs to the class of WNT proteins that activate the “noncanonical" pathway. The predicted size of human WNT-5a is a monomeric protein containing 357 amino acid residues. Due to glycosylation, it migrates at an apparent molecular weight of ~45 kDa on SDS-PAGE under non-reducing conditions. StemRD’s product is expressed from a human cell line in animal-free medium, and purified with a proprietary process that is distinct from the published method.
? Purity: Greater than 85% as determined by SDS-PAGE and HPLC analysis
? Biological Activity: The activity was determined by using a TCF reporter gene assay in HEK293 cells co-transfected with Frizzled-4 and LRP- 5. WNT-5a activates (instead of inhibits) the TCF reporter gene in this assay (MilkelsAJ, et al., PLoS Biol, 4:e115, 2006). This activation mode is utilized because activation assays are generally more reliable than inhibition assays, as they are less prone to any non-specific inhibitory contamination in the preparation.
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StemRD MGro-500 MSC medium,chemically-defined,间充质干细胞培养基 500mL/瓶
LONZA 04-418Q X-VIVO 15 无血清培养基 1L/瓶
Life Technology 087-0112DK AIM-V? CTS? 无血清细胞培养基(细胞治疗级) 1000mL/瓶
Pall 15950-017 Ultroser Serum Substitute 血清替代品 20mL/瓶
LONZA 12-725F UltraCULTURE Serum-free Medium无血清培养基 500mL/瓶
BD 762165 PAXgene Blood RNA Tube RNA样本收集管 100管/盒
LONZA 50150 SeaKem? Gold Agarose 金琼脂糖粉 125g/瓶
LONZA CC-3162 EGM-2 BulletKit( CC-3156 & CC-4176 )套装 Kit/套
Nunc 159910 175cm2易用培养瓶,PS,已灭菌,瓶盖:过滤盖 30个/箱
LONZA LT07-318 MycoAlert? Mycoplasma Detection Kit支原体检测试剂盒 100Tests/盒
LONZA LT07-418 MycoAlert? Mycoplasma Detection Kit支原体检测试剂盒 50Tests/盒
Lonza 50-650U Kinetic-QCL 192 Test Kit内毒素检测试剂盒 192T/盒
Corning 431080 175cm⒉细胞培养瓶,Angled颈,透气盖 50个/箱
Corning 8388 125mL储液瓶 Storage Bottles,PS,45mm瓶颈,带盖,灭菌,独立包装 24个/箱
Corning 430659 2ml冻存管, 聚丙烯,外旋盖, 可自立圆底 500个/箱
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Corning 3292 175cm2 CellBIND Surface培养瓶,已灭菌,透气盖 50个/箱
BD-Falcon 352360 Cell Strainer, 100μm, Yellow 100um细胞滤网 50个/箱
BD Falcon 352070 50mL锥形离心管,高透明度聚丙烯,袋装 500支/箱
BD Falcon 352096 15mL锥形离心管,高透明度聚丙烯,袋装 500支/箱
BD BioCoat 354234 Matrigel Basement Membrane Matrix(标准型) 10mL/瓶
Cygnus F550 CHO HCP ELISA Kit,3G宿主蛋白检测试剂盒 96T/盒
Cygnus F410 E.coli HCP ELISA Kit 大肠杆菌 96T/盒
Nalgene 5100-0001 Cryo 1℃ Freezing Container梯度降温冻存盒 1个/箱
Nalgene 5026-0909 CryoBoxes 冻存盒 9X9 阵列,管瓶1.2/2.0mL 24个/箱
Nalgene 2250-0130 50L 可高温高压操作的细口大瓶(带手柄) 1个/箱
Nalgene 2319-0050 20L 可高温高压灭菌的细口大瓶(带放水口) 1个/箱
Hyclone SH30084.03 FBS 优等澳洲胎牛血清 500mL/瓶
PeproTech 100-18B-500 Recombinant Human FGF-basic (154 a.a.)因子 500ug/支
Parafilm PM-996 Parafilm? M Laboratory Film 封口膜 12卷/箱